Assuntos
Enterococcus faecium , Infecções por Bactérias Gram-Positivas , Animais , Enterococcus faecium/genética , Animais de Estimação , Brasil/epidemiologia , Antibacterianos/farmacologia , Células Clonais , Infecções por Bactérias Gram-Positivas/epidemiologia , Infecções por Bactérias Gram-Positivas/veterinária , Proteínas de Bactérias , Carbono-Oxigênio Ligases , Testes de Sensibilidade MicrobianaRESUMO
Mangrove ecosystems are hotspots of biodiversity, but have been threatened by anthropogenic activities. Vancomycin-resistant enterococci (VRE) are nosocomial bacteria classified as high priority by the World Health Organization (WHO). Herein, we describe the identification and genomic characteristics of a vancomycin-resistant Enterococcus faecalis strain isolated from a highly impacted mangrove ecosystem of the northeastern Brazilian, in 2021. Genomic analysis confirmed the existence of the transposon Tn1546-vanA and clinically relevant antimicrobial resistance genes, such as streptogramins, tetracycline, phenicols, and fluoroquinolones. Virulome analysis identified several genes associated to adherence, immune modulation, biofilm, and exoenzymes production. The UFSEfl strain was assigned to sequence type (ST9), whereas phylogenomic analysis with publicly available genomes from a worldwide confirmed clonal relatedness with a hospital-associated Brazilian clone. Our findings highlight the successful expansion of hospital-associated VRE in a mangrove area and shed light on the need for strengthening genomic surveillance of WHO priority pathogens in these vital ecosystems.
Assuntos
Ecossistema , Enterococcus faecium , Infecções por Bactérias Gram-Positivas , Enterococos Resistentes à Vancomicina , Humanos , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Brasil/epidemiologia , Células Clonais , Enterococcus faecalis/genética , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Infecções por Bactérias Gram-Positivas/epidemiologia , Infecções por Bactérias Gram-Positivas/microbiologia , Testes de Sensibilidade Microbiana , Vancomicina , Resistência a Vancomicina/genética , Enterococos Resistentes à Vancomicina/genética , Infecção Hospitalar/microbiologiaRESUMO
Methicillin-resistant staphylococci have become leading cause of infectious diseases in humans and animals, being categorized as high priority pathogens by the World Health Organization. Although methicillin-resistant Staphylococcus sciuri (recently moved to Mammaliicoccus sciuri) has been widely reported in companion animals, there is scarce information regarding their clinical impact and genomic features. Herein, we reported the occurrence and genomic characteristics of methicillin-resistant M. sciuri recovered from fatal infections in pets admitted to an intensive care unit of a veterinary hospital, in Brazil. Two M. sciuri strains were isolated from bronchoalveolar lavage samples collected from dog (strain SS01) and cat (strain SS02) presenting with sepsis and acute respiratory distress syndrome. Both isolates displayed a multidrug-resistant profile, whereas whole-genome sequencing analysis confirmed the presence of the mecA gene, along to genetic determinant conferring resistance to macrolides, streptogramins, aminoglycosides, and trimethoprim. For both strains, the mec and crr gene complex shared high identity (≥97%) with analogue sequences from a M. sciuri isolated from a human wound infection, in the Czech Republic. Strains were assigned to the sequence type ST52 and the novel ST74. Phylogenomic analysis revealed a broad host range association of these strains with several hosts and sources, including humans, animals, food, and the environment through different years and geographic locations. Our findings demonstrate that infections caused by mecA-positive M. sciuri strains can be a serious threat for veterinary intensive care patients and the medical staff, with additional implications for One Health approaches.
Assuntos
Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Aminoglicosídeos , Animais , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Cães , Genômica , Humanos , Unidades de Terapia Intensiva , Macrolídeos , Resistência a Meticilina , Staphylococcus aureus Resistente à Meticilina/genética , Testes de Sensibilidade Microbiana , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/veterinária , Staphylococcus , Estreptograminas , TrimetoprimaAssuntos
Doenças do Gato , Doenças do Cão , Animais , Antibacterianos/farmacologia , Proteínas de Bactérias , Doenças do Gato/tratamento farmacológico , Gatos , Doenças do Cão/tratamento farmacológico , Cães , Enterococcus , Testes de Sensibilidade Microbiana/veterinária , Vancomicina , Organização Mundial da SaúdeRESUMO
We report the occurrence and genomic features of multidrug-resistant vancomycin-resistant Enterococcus faecium vanA belonging to a novel sequence type (designated ST1336), carrying a Tn1546-like element, in marine brown mussels (Perna perna) from anthropogenically affected coastal waters of the Atlantic coast of Brazil, highlighting a potential source of dissemination for related ecosystems, with additional consequences for seafood safety and quality.
Assuntos
Enterococcus faecium/efeitos dos fármacos , Enterococcus faecium/isolamento & purificação , Perna (Organismo)/microbiologia , Resistência a Vancomicina/genética , Animais , Proteínas de Bactérias/genética , Brasil , Carbono-Oxigênio Ligases/genética , Elementos de DNA Transponíveis , Ecossistema , Enterococcus faecium/genética , Humanos , Testes de Sensibilidade MicrobianaRESUMO
OBJECTIVES: Genomic surveillance studies monitoring the dissemination of multidrug-resistant Enterobacteriaceae in polluted aquatic ecosystems are urgently required. The aim of this study was to report the draft genome sequence of an MCR-1 and CTX-M-8 co-producing Escherichia coli isolated from a polluted mangrove ecosystem in Northeast Brazil. METHODS: Total genomic DNA was sequenced on an Illumina NextSeq platform and was assembled using CLC Genomics Workbench. The whole-genome sequence was evaluated through bioinformatics tools available from the Center of Genomic Epidemiology as well as additional in silico analysis. RESULTS: The genome size was calculated at 5089467bp, comprising a total of 5068 protein-coding sequences. The strain was assigned to sequence type 58 (ST58) and revealed the presence of mcr-1, blaCTX-M-8 and other clinically significant genes responsible for conferring resistance to colistin, ß-lactams, trimethoprim and quinolones. In addition, genes conferring resistance to silver (silR) and quaternary ammonium compounds (sugE) were identified. CONCLUSION: These data provide valuable information for comparative genomic analysis regarding the dissemination of MCR-1-producing E. coli at the human-animal-environment interface.
Assuntos
Proteínas de Escherichia coli/genética , Escherichia coli/isolamento & purificação , Genoma Bacteriano , Microbiologia da Água , beta-Lactamases/genética , Antibacterianos/farmacologia , Brasil , Farmacorresistência Bacteriana , Ecossistema , Escherichia coli/efeitos dos fármacos , Escherichia coli/enzimologia , Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Tamanho do Genoma , Genômica , Áreas Alagadas , Sequenciamento Completo do Genoma , beta-Lactamases/metabolismoRESUMO
We report changes in the molecular epidemiology of vanA-containing Enterococcus during the intra and interhospital spread of high-risk clones, in Southeastern Brazil. While VRE faecalis predominated during 1998 to 2006, a reversal has been observed in the last years, where VRE faecium belonging to ST114, ST203, ST412, ST478 and ST858 have become endemic.
Assuntos
Infecção Hospitalar/epidemiologia , Infecções por Bactérias Gram-Positivas/epidemiologia , Antibacterianos/uso terapêutico , Proteínas de Bactérias/genética , Brasil/epidemiologia , Infecção Hospitalar/microbiologia , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/genética , Enterococcus faecium/efeitos dos fármacos , Infecções por Bactérias Gram-Positivas/microbiologia , Hospitais , Humanos , Testes de Sensibilidade Microbiana/métodos , Epidemiologia Molecular/métodos , Resistência a Vancomicina/efeitos dos fármacosAssuntos
Farmacorresistência Bacteriana , Enterococcus faecalis/enzimologia , Enterococcus faecalis/isolamento & purificação , Infecções por Bactérias Gram-Positivas/veterinária , tRNA Metiltransferases/genética , Animais , Antibacterianos/farmacologia , Brasil , DNA Bacteriano/química , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Enterococcus faecalis/classificação , Enterococcus faecalis/genética , Infecções por Bactérias Gram-Positivas/microbiologia , Tipagem Molecular , Reação em Cadeia da Polimerase , Proteínas Ribossômicas/genética , Análise de Sequência de DNA , SuínosAssuntos
Proteínas de Bactérias/genética , Carbono-Oxigênio Ligases/genética , Galinhas/microbiologia , Enterococcus faecalis/classificação , Enterococcus faecium/classificação , Carne/microbiologia , Animais , Antibacterianos , Brasil , Contaminação de Alimentos , Microbiologia de Alimentos , Testes de Sensibilidade MicrobianaAssuntos
Proteínas de Bactérias/genética , Carbono-Oxigênio Ligases/genética , Enterococcus faecium/classificação , Enterococcus faecium/isolamento & purificação , Genótipo , Rios/microbiologia , Enterococos Resistentes à Vancomicina/classificação , Enterococos Resistentes à Vancomicina/isolamento & purificação , Antibacterianos/farmacologia , Brasil , Enterococcus faecium/genética , Hospitais , Humanos , Testes de Sensibilidade Microbiana , Tipagem Molecular , Enterococos Resistentes à Vancomicina/genéticaRESUMO
We report the whole-genome sequence (WGS) of an in vitro susceptible derivative revertant mutant from a bloodstream isolate involved in a nosocomial outbreak in Brazil. The WGS comprises 2.5 Mb with 2,500 protein-coding sequences, 16rRNA genes, and 60 tRNA genes.